Journal: iScience

Article Title: Hedgehog signaling is required for endometrial remodeling and myometrial homeostasis in the cycling mouse uterus

doi: 10.1016/j.isci.2023.107993

Figure Lengend Snippet:

Article Snippet: Hematoxylin and Eosin Stain kit , StatLab , Cat#: KTMTRLT.

Techniques: Staining, H&E Stain, Reverse Transcription

Journal: International Journal of Molecular Sciences

Article Title: Endothelial Autocrine Signaling through CXCL12/CXCR4/FoxM1 Axis Contributes to Severe Pulmonary Arterial Hypertension

doi: 10.3390/ijms22063182

Figure Lengend Snippet: Foxm1 deletion in ECs attenuated pulmonary vascular remodeling in Egln1 Tie2Cre mice. ( A , B ) Representative images of Russell−Movat pentachrome staining showing the reduction of pulmonary wall thickness in Egln1/Foxm1 Tie2Cre mice ( Efoxm1 ). (CKO, n = 4; Efoxm1, n = 4). ( C , D ) Immunostaining against α -SMA demonstrated attenuation of pulmonary vascular remodeling including neointima and muscularization of distal PAs in Egln1/Foxm1 Tie2Cre mice. Red arrows indicate the muscularization of distal PAs. (CKO, n = 5; Efoxm1, n = 5). V = vessel. Student t -test, * p < 0.05. Scale bar = 50 μm ( A , C ).

Article Snippet: Similarly, after antigen retrieval, mouse lung sections were incubated with anti-α-SMA (Cat #ab5694, 1:300, Abcam, Cambridge, UK) at 4 °C overnight and then incubated with Alexa 594 conjugated anti-rabbit IgG (Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 1 h. For histology assessment, lung sections were dewaxed, dehydrated, and stained with a Russell−Movat pentachrome staining kit (Cat #KTRMP, StatLab, McKinney, TX, USA) according to the manufacturer’s instructions.

Techniques: Staining, Immunostaining

Journal: Bioactive Materials

Article Title: Mesenchymal stem cell activity across a graded scaffold-hydrogel composite biomaterial for tendon-to-bone enthesis repair

doi: 10.1016/j.bioactmat.2025.07.017

Figure Lengend Snippet: Histological evaluation of triphasic scaffold structure and matrix remodeling. FFPE sections of hMSC-seeded triphasic scaffolds (n = 2) at both Day 1 and Day 21 were stained with either trichrome, alizarin red, or alcian blue staining. Representative images are provided of (A) a scan of a full scaffold (trichrome only), (B) the osseous region, (C) the tendinous region, and (D) the interfacial region.

Article Snippet: Samples were stained by one of four staining procedures: hematoxylin/eosin (H&E, SL200 & SL201, StatLab), alizarin red (Cat# 2003999, EMD Millipore Corp.), trichrome (Cat# ab150686, Abcam), or alcian blue (pH 2.5, Cat# KTABP2.5, StatLab) with staining procedures followed according to manufacturer recommendation.

Techniques: Staining

Journal: Nature protocols

Article Title: Simultaneous isolation of high-quality DNA, RNA, miRNA and proteins from tissues for genomic applications

doi: 10.1038/nprot.2013.141

Figure Lengend Snippet: Comparison of mutation detection rates among different genomic studies in ccRCC.

Article Snippet: Ethanol, 100%, for histology (Koptec, cat. no. V1001) and for nucleic acid extraction (Koptec, cat. no. 1016) Xylene (StatLab, cat. no. 8400-1) Paraffin (Paraplast Plus tissue embedding medium, McCormick Scientific, cat. no. 39502004) Hematoxylin (StatLab, cat. no. SL95-16) High Def (StatLab, cat. no. SL103) Bluing reagent (Thermo Scientific, cat. no. 7301) Eosin Y (StatLab, cat. no. SL98-16) Cytoseal 60 (Thermo Scientific, cat. no. 8310-4) QIAshredder (Qiagen, cat. no. 79654) AllPrep DNA/RNA mini kit (Qiagen, cat. no. 80204) mir Vana miRNA isolation kit (Life Technologies (Ambion), cat. no. AM1560) Nuclease-free water (Life Technologies (Ambion), cat. no. AM9937) Tris base (Research Products International, cat. no. T60040-5000) HCl (Fisher, cat. no. A144-500) NaCl (Research Products International, cat. no. S23020-5000) Igepal (Sigma, cat. no. I8896) Complete protease inhibitor cocktail (Roche, cat. no. 11873580001) Aprotinin (Research Products International, cat. no. A20550) Leupeptin (Research Products International, cat. no. L22035) Pepstatin A (Research Products International, cat. no. {"type":"entrez-protein","attrs":{"text":"P30100","term_id":"231899","term_text":"P30100"}} P30100 ) Benzamidine (Sigma, cat. no. 12072) Phenylmethanesulfonyl fluoride (PMSF; Research Products International, cat. no. {"type":"entrez-protein","attrs":{"text":"P20270","term_id":"2851503","term_text":"P20270"}} P20270 ) NaF (Sigma, cat. no. S7920) Imidazole (Sigma, cat. no. I5513) Sodium molybdate (Sigma, cat. no. 243655) Sodium orthovanadate (Sigma, cat. no. S6508) Microcystin (Millipore, cat. no. 475815) Acetone (Millipore, cat. no. AX0120-8) Bio-Rad Protein Assay (Bio-Rad, cat. no. 500-0002)

Techniques: Comparison, Mutagenesis, DNA Extraction, Sequencing, Extraction, Subcloning